diff --git a/Makefile b/Makefile
index f350ecb..9160860 100644
--- a/Makefile
+++ b/Makefile
@@ -702,6 +702,10 @@ check-symbols: startup_files libc
 	    | sed -e 's/__GNUC_VA_LIST $$/__GNUC_VA_LIST 1/' \
 	    | grep -v '^#define __\(BOOL\|INT_\(LEAST\|FAST\)\(8\|16\|32\|64\)\|INT\|LONG\|LLONG\|SHRT\)_WIDTH__' \
 	    | grep -v '^#define __GCC_HAVE_SYNC_COMPARE_AND_SWAP_\(1\|2\|4\|8\)' \
+	    | grep -v '^#define NDEBUG' \
+	    | grep -v '^#define __OPTIMIZE__' \
+	    | grep -v '^#define assert' \
+	    | grep -v '^#define __NO_INLINE__' \
 	    > "$(SYSROOT_SHARE)/predefined-macros.txt"
 
 	# Check that the computed metadata matches the expected metadata.
diff --git a/expected/wasm32-wasi-threads/predefined-macros.txt b/expected/wasm32-wasi-threads/predefined-macros.txt
index 8b37315..0fca3e4 100644
--- a/expected/wasm32-wasi-threads/predefined-macros.txt
+++ b/expected/wasm32-wasi-threads/predefined-macros.txt
@@ -1119,7 +1119,6 @@
 #define NAN (0.0f/0.0f)
 #define NBBY 8
 #define NCARGS 131072
-#define NDEBUG 1
 #define ND_NA_FLAG_OVERRIDE 0x00000020
 #define ND_NA_FLAG_ROUTER 0x00000080
 #define ND_NA_FLAG_SOLICITED 0x00000040
@@ -2685,7 +2684,6 @@
 #define __OPENCL_MEMORY_SCOPE_SUB_GROUP 4
 #define __OPENCL_MEMORY_SCOPE_WORK_GROUP 1
 #define __OPENCL_MEMORY_SCOPE_WORK_ITEM 0
-#define __OPTIMIZE__ 1
 #define __ORDER_BIG_ENDIAN__ 4321
 #define __ORDER_LITTLE_ENDIAN__ 1234
 #define __ORDER_PDP_ENDIAN__ 3412
@@ -3112,7 +3110,6 @@
 #define and_eq &=
 #define asin(x) __tg_real_complex(asin, (x))
 #define asinh(x) __tg_real_complex(asinh, (x))
-#define assert(x) (void)0
 #define atan(x) __tg_real_complex(atan, (x))
 #define atan2(x,y) __tg_real_2(atan2, (x), (y))
 #define atanh(x) __tg_real_complex(atanh, (x))
diff --git a/expected/wasm32-wasi/predefined-macros.txt b/expected/wasm32-wasi/predefined-macros.txt
index f061007..d6cae22 100644
--- a/expected/wasm32-wasi/predefined-macros.txt
+++ b/expected/wasm32-wasi/predefined-macros.txt
@@ -1119,7 +1119,6 @@
 #define NAN (0.0f/0.0f)
 #define NBBY 8
 #define NCARGS 131072
-#define NDEBUG 1
 #define ND_NA_FLAG_OVERRIDE 0x00000020
 #define ND_NA_FLAG_ROUTER 0x00000080
 #define ND_NA_FLAG_SOLICITED 0x00000040
@@ -2648,7 +2647,6 @@
 #define __OPENCL_MEMORY_SCOPE_SUB_GROUP 4
 #define __OPENCL_MEMORY_SCOPE_WORK_GROUP 1
 #define __OPENCL_MEMORY_SCOPE_WORK_ITEM 0
-#define __OPTIMIZE__ 1
 #define __ORDER_BIG_ENDIAN__ 4321
 #define __ORDER_LITTLE_ENDIAN__ 1234
 #define __ORDER_PDP_ENDIAN__ 3412
@@ -3073,7 +3071,6 @@
 #define and_eq &=
 #define asin(x) __tg_real_complex(asin, (x))
 #define asinh(x) __tg_real_complex(asinh, (x))
-#define assert(x) (void)0
 #define atan(x) __tg_real_complex(atan, (x))
 #define atan2(x,y) __tg_real_2(atan2, (x), (y))
 #define atanh(x) __tg_real_complex(atanh, (x))